2. Signaling Pathways
  3. Cell Cycle/DNA Damage
  4. DNA/RNA Synthesis

DNA/RNA Synthesis

DNA/RNA Synthesis

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RNA synthesis, which is also called DNA transcription, is a highly selective process. Transcription by RNA polymerase II extends beyond RNA synthesis, towards a more active role in mRNA maturation, surveillance and export to the cytoplasm.

Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, with DNA ligase creating the final phosphodiester bond to fully repair the DNA.DNA ligases discriminate against substrates containing RNA strands or mismatched base pairs at positions near the ends of the nickedDNA. Bleomycin (BLM) exerts its genotoxicity by generating free radicals, whichattack C-4′ in the deoxyribose backbone of DNA, leading to opening of the ribose ring and strand breakage; it is an S-independentradiomimetic agent that causes double-strand breaks in DNA.

First strand cDNA is synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. The remaining overhangs are converted into blunt ends using exonuclease/polymerase activity. After adenylation of the 3′ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare the samples for hybridization. Cell cycle and DNA replication are the top two pathways regulated by BET bromodomain inhibition. Cycloheximide blocks the translation of mRNA to protein.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-B0016S
    Capecitabine-d11
    		Inhibitor 99%
    Capecitabine-d11 is the deuterium labeled Capecitabine. Capecitabine is an oral proagent that is converted to its active metabolite, 5-FU, by thymidine phosphorylase.
    Capecitabine-d<sub>11</sub>
  • HY-125650
    Pseudouridimycin
    		Inhibitor
    Pseudouridimycin (PUM) is an antibiotic that selectively inhibits bacterial RNA polymerase (RNAP), with an IC50 of about 0.1 μM and MICs of 4-6 μg/mL. Pseudouridimycin is a C-nucleoside analogue that's effective against both Gram-negative and Gram-positive bacteria. Pseudouridimycin inhibits bacterial growth in vitro and shows activity in a mouse model of purulent streptococcal peritonitis.
    Pseudouridimycin
  • HY-N6845
    3-Isomangostin
    		Inhibitor 98.99%
    3-Isomangostin, extracted from Garciniamangostana.L. shell, is a potent MutT homologue 1 (MTH1) inhibitor with an IC50 value of 52 nM. 3-Isomangostin would be an attractive chemical tool for the development of anticancer agents.
    3-Isomangostin
  • HY-169090
    TS-002902
    		Inhibitor 98.29%
    TS-002902 is a small molecule TUT4/7 inhibitor with antiproliferative activity. TS-002902 is promising for research of cancers.
    TS-002902
  • HY-75800
    Lomibuvir
    		Inhibitor 99.71%
    Lomibuvir (VX-222), a selective, non-nucleoside polymerase inhibitor, targets thumb pocket 2 of the HCV NS5B polymerase (RdRp) with a Kd of 17 nM. Lomibuvir inhibits the 1b/Con1 HCV subgenomic replicon with an EC50 of 5.2 nM. Lomibuvir preferentially inhibits elongative RNA synthesis rather than de novo-initiated RNA synthesis.
    Lomibuvir
  • HY-169091
    TS-002266
    		Inhibitor 98.99%
    TS-002266 is a small molecule TUT4/7 inhibitor with anti-leukemic effects. TS-002266 is promising for research of cancers.
    TS-002266
  • HY-101813
    Laflunimus
    		Inhibitor 99.70%
    Laflunimus (HR325) is an immunosuppressive agent and an analogue of the Leflunomide-active metabolite A77 1726. Laflunimus is an orally active inhibitor of dihydroorotate dehydrogenase (DHODH). Laflunimus suppresses immunoglobulin (Ig) secretion, with IC50 values of 2.5 and 2 µM for IgM and IgG, respectively. Laflunimus also is a prostaglandin endoperoxide H synthase (PGHS) -1 and -2 inhibitor.
    Laflunimus
  • HY-136373
    Metazachlor
    		99.76%
    Metazachlor (BAS 479H) is a herbicide belonging to the chloroacetamides class. Metazachlor can inhibit the synthesis of very long chain fatty acids during the germination and emergence of weed seeds, thereby interfering with cell division and tissue differentiation, and thus hindering the normal growth and development of weeds.
    Metazachlor
  • HY-107790
    5-Methoxyflavone
    		Inhibitor 99.97%
    5-Methoxyflavone, belonged to Flavonoid family, is a DNA polymerase-beta inhibitor and neuroprotective agent against beta-amyloid toxicity. possess central nervous system (CNS) depressant effect mediated through the ionotropic GABAA receptors.
    5-Methoxyflavone
  • HY-143498
    ERCC1-XPF-IN-1
    		Inhibitor 98.05%
    ERCC1-XPF-IN-1 is a potent and high-affinity ERCC1-XPF inhibitor with IC50 value of 0.49 μM. ERCC1-XPF-IN-1 has the capacity to potentiate the cytotoxicity effect of UV radiation and inhibiting DAN repair, by the inhibition of removal of CPDs, and cyclophosphamide toxicity to colorectal cancer cells.
    ERCC1-XPF-IN-1
  • HY-B0220D
    Erythromycin thiocyanate
    		Inhibitor
    Erythromycin thiocyanate is a macrolide antibiotic produced by actinomycete Streptomyces erythreus with a broad spectrum of antimicrobial activity. Erythromycin thiocyanate binds to bacterial 50S ribosomal subunits and inhibits RNA-dependent protein synthesis by blockage of transpeptidation and/or translocation reactions, without affecting synthesis of nucleic acid[1][2]. Erythromycin thiocyanate also exhibits antitumor and neuroprotective effect in different fields of research[3][4].
    Erythromycin thiocyanate
  • HY-107372
    Uridine triphosphate
    Uridine triphosphate (UTP) is a pyrimidine nucleoside triphosphate that is used as a substrate to synthesize RNA or as an energy source in metabolic reactions. Uridine triphosphate activates membrane-bound P2Y2 receptors.
    Uridine triphosphate
  • HY-W177546
    CD00509
    		Inhibitor ≥98.0%
    CD00509 is a tyrosyl-DNA phosphodiesterase (PDE) Tdp1 inhibitor with an IC50 of 0.71 μM. CD00509 increases DNA damage and promotes apoptosis of MCF-7 cells. CD00509 can sensitize breast cancer cells and wild-type murine embryonic fibroblasts (MEFs) to Camptothecin (CPT) (HY-16560). CD00509 can be used for the study of cancers such as breast cancer.
    CD00509
  • HY-138616S4
    dGTP-13C10,15N5 dilithium
    		98.2%
    dGTP-13C10,15N5 (2'-Deoxyguanosine-5'-triphosphate-13C10,15N5) dilithium is 13C and 15N-labeled dGTP (HY-138616). dGTP (2'-Deoxyguanosine-5'-triphosphate), a guanosine nucleotide, can be used in deoxyribonucleic acid synthesis. Guanosine nucleotides (GDP, GTP, dGDP, and dGTP) are highly susceptible to oxidative damage to 8-oxo-GDP (8-O-GDP), 8-O-dGTP, 8-O-GTP, and 8-O-dGTP.
    dGTP-<sup>13</sup>C<sub>10</sub>,<sup>15</sup>N<sub>5</sub> dilithium
  • HY-112062
    POL1-IN-1
    		Inhibitor 98.87%
    POL1-IN-1 is a RNA polymerase 1 (POL1, also known as Pol I) inhibitor with an IC50 of less than 0.5 uM. POL1-IN-1 inhibits ribosome biogenesis by inhibiting POL1 transcription.
    POL1-IN-1
  • HY-B0152S
    Adenine-d
    		Chemical 99.90%
    Adenine-d is the deuterium labeled Adenine. Adenine (6-Aminopurine), a purine, is one of the four nucleobases in the nucleic acid of DNA. Adenine acts as a chemical component of DNA and RNA. Adenine also plays an important role in biochemistry involved in cellular respiration, the form of both ATP and the cofactors (NAD and FAD), and protein synthesis.
    Adenine-d
  • HY-N4321
    Glucotropaeolin potassium
    		99.40%
    Glucotropaeolin potassium (Benzylglucosinolate potassium) , a glucosinolate contained in cruciferous vegetables, causes a moderate decrease in spontaneous DNA damage in animals.
    Glucotropaeolin potassium
  • HY-116364B
    AZT triphosphate tetraammonium
    		Inhibitor 99.45%
    AZT triphosphate (3'-Azido-3'-deoxythymidine-5'-triphosphate) tetraammonium is an active triphosphate metabolite of Zidovudine (AZT). AZT triphosphate tetraammonium exhibits antiretroviral activity and inhibits replication of HIV. AZT triphosphate tetraammonium also inhibits the DNA polymerase of HBV. AZT triphosphate tetraammonium activates the mitochondria-mediated apoptosis pathway.
    AZT triphosphate tetraammonium
  • HY-121834
    RNase L-IN-2
    		98.63%
    RNase L-IN-2 (compound 2) is an activtor of RNase L with EC50 value of 22 μM.
    RNase L-IN-2
  • HY-155583A
    RNase L-IN-1 trihydrochloride
    		99.56%
    RNase L-IN-1 (compound 17a) trihydrochloride is an inhibitor of RNase L or ribonuclease L. RNase L degrades RNA to prevent viral replication and mediates innate immune responses and inflammation.
    RNase L-IN-1 trihydrochloride
Cat. No. Product Name / Synonyms Application Reactivity